Antihyperlipidemic and Hepatoprotective Effects of Different Fractions of Brassica Oleracea in Alloxan Induced Diabetic Rats

The study was undertaken to investigate the hypolipidemic and hepatoprotective effects of the different fractions (Petroleum ether, ethyl acetate and chloroform) of ethanolic extract of Brassica oleracea. The different fractions of extract were administered intraperitoneally as a single dose of 150 mg/kg body weight to alloxan induced diabetic rats and found to reduce blood lipid level (Total cholesterol and Triglycerides) significantly (p<0.05). The plant fractions also exhibited correction of altered biochemical parameters viz., SGOT and SGPT levels in diabetic rats. The effect of plant fractions were compared with standard drug metformin. The phytochemical screening tests indicated that the different constituents such as saponins, tannins, triterpenes, alkaloids and flavonoids etc. were present in the plant which has hypolipidemic and hepatoprotective properties. Thus, this study indicates that various fractions (Petroleum ether, ethyl acetate and chloroform) of the ethanolic extract of Brassica oleracea have favorable effect in hypolipidemic and hepatoprotective activities. INTRODUCTION: Hyperlipidemia mainly an increased level of total cholesterol, triglycerides and low-density lipoprotein cholesterol along with a decrease in highdensity lipoprotein cholesterol, is the predictor of coronary artery disease, fatty liver disease, and carcinogenesis, which is associated with the formation of reactive oxygen species . Hyperlipidemia is a predominant risk factor for cardiovascular diseases . Hyperlipidemia is an important risk factor in the initiation and progression of atherosclerotic impasse . Hypercholesterolemia and hypertriglyceridemia are common complications of diabetes mellitus in addition to hyperglycemia. The frequency of hyperlipidemia in diabetes is indeed very high depending on the type of diabetes and its degree of control. Diabetes mellitus is one of the oldest diseases affecting millions of people all over the world . Diabetes is a metabolic disorder featured by hyperglycemia as well as hyperlipidemia. The alterations in carbohydrate, fat and protein metabolism associated with absolute or relative deficiency of insulin secretion and insulin action . Although numerous oral hypoglycemic and